Characterization of Oligomer Formation of Surfactant Protein-D (SP-D) Using AF4-MALLS
Authors: Ryan R. Manning, Ryan E. Holcomb, Derrick S. Katayama, Giovanni M. Pauletti, Shawn N. Grant, Jan S. Rosenbaum, and Mark Cornell Manning Characterization of Oligomer Formation of Surfactant Protein-D (SP-D) Using AF4-MALLS Abstract: Background: Surfactant protein-S (SP-D) is a naturally occurring lung protein with the potential to treat pulmonary infections. A recombinant surfactant protein-D (SP-D) has …
Commentary: Pattern Recognition Proteins: First Line of Defense Against Coronaviruses
Authors: Michael DePietro and Marc Salzberg, Airway Therapeutics, Marietta, GA, United States A Commentary on Pattern Recognition Proteins: First Line of Defense Against Coronaviruses By Labarrere CA and Kassab GS (2021). Front. Immunol. 12:652252. doi: 10.3389/fimmu.2021.652252 Introduction Labarrere and Kassab recently wrote an informative paper that includes a very interesting overview of the role Surfactant …
Full-Length Recombinant hSP-D Binds and Inhibits SARS-CoV-2
SARS-CoV-2 infection of host cells is driven by binding of the SARS-CoV-2 spike-(S)-protein to lung type II pneumocytes, followed by virus replication. Surfactant protein SP-D, member of the front-line immune defense of the lungs, binds glycosylated structures on invading pathogens such as viruses to induce their clearance from the lungs. The objective of this study …
Surfactant Protein D and Bronchopulmonary Dysplasia: A New Way to Approach an Old Problem
Surfactant protein D and bronchopulmonary dysplasia: a new way to approach an old problem Surfactant protein D (SP-D) is a collectin protein synthesized by alveolar type II cells in the lungs. SP-D participates in the innate immune defense of the lungs by helping to clear infectious pathogens and modulating the immune response. SP-D has shown …
Pulmonary Surfactant and Drug Delivery: An Interface-Assisted Carrier to Deliver Surfactant Protein
Frontiers | Pulmonary Surfactant and Drug Delivery: An Interface-Assisted Carrier to Deliver Surfactant Protein SP-D Into the Airways | Bioengineering and Biotechnology (frontiersin.org) This work is focused on the potential use of pulmonary surfactant to deliver full-length recombinant human surfactant protein SP-D (rhSP-D) using the respiratory air-liquid interface as a shuttle. Surfactant protein D (SP-D) …
EVALUATION OF RECOMBINANT HUMAN SP-D IN THE RAT PREMATURE LUNG MODEL
The lungs of premature and term babies are structurally different from the adult lungs. Preterm lungs are underdeveloped, non-compliant in terms of breathing, often need mechanical ventilation and these patients commonly develop syndromes as a consequence of their prematurity, such as bronchopulmonary dysplasia (BPD). Surfactant protein SP-D could be a therapy for BPD. However, there …
Surfactant Protein-D Regulates Surfactant Phospholipid Homeostasis in Vivo
Surfactant protein D (SP-D) is a 43-kDa member of the collectin family of collagenous lectin domain-containing proteins that is expressed in epithelial cells of the lung. The SP-D gene was targeted by homologous recombination in embryonic stem cells that were used to produce SP-D (6) and SP-D (2/2) mice. Both SP-D (2/2) and SP-D (6) …
Surfactant metabolism in SP-D gene-targeted mice
Mice with surfactant protein (SP)-D deficiency have three to four times more surfactant lipids in air spaces and lung tissue than control mice. We measured multiple aspects of surfactant metabolism and function to identify abnormalities resulting from SP-D deficiency. Relative to saturated phosphatidylcholine (Sat PC), SP-A and SP-C were decreased in the alveolar surfactant and …
Distinct Effects of Surfactant Protein A or D Deficiency During Bacterial Infection on the Lung
Mice lacking surfactant protein (SP)-A (SP-A−/−) or SP-D (SP-D−/−) and wild-type mice were infected with group B streptococcus or Haemophilus influenzae by intratracheal instillation. Although decreased killing of group B streptococcus and H. influenzae was observed in SP-A−/− mice but not in SP-D−/− mice, deficiency of either SP-A or SP-D was associated with increased inflammation …
Surfactant protein A recruits neutrophils into the lungs of ventilated preterm lambs.
We tested the effects of surfactant protein A (SP-A) on inflammation and surfactant function in ventilated preterm lungs. Preterm lambs of 131 d gestation were ventilated for 15 min to initiate a mild inflammatory response, and were then treated with 100 mg/ kg recombinant human SP-C surfactant or with the same surfactant supplemented with 3 …
